Hodophilus pseudoglabripes

Hodophilus pseudoglabripes S. M Tang & S.H. Li

Basidiomata small to medium-sized. Pileus 11–25 mm diam., hemispherical at first, then convex

to plano-convex, often with a slight depression at the center, surface smooth when young, becoming slightly striate near margin and flocculose with age, when wet translucently striated halfway to the disc, hygrophanous, white (1A1) to yellowish white (1A2) when young, later greyish yellow (1B4) to light orange (5A5), when dry uniformly pale at center. Pileus context up to 1.5 mm thick, white (1A1). Lamellae number 16–20, white (1A1) when young, light orange (5A5) to greyish orange (5B5) when old, 3–4 mm wide, lamellullae frequent, in (1–) 2 tiers. Stipe 34–64 × 2–3 mm, surface smooth, white (1A1), central, usually flexuous, hollow, and narrowing downwards, becoming greyish yellow (2B3) near the base, hollow when mature. Odor strong earthy. Basidiospores (103/5/2) (4.0–) 4.3–6.2 (–6.3) × (3.4–) 3.5–5.5 (–5.6) μm, av. 5.2–4.6 μm, Q (length/width) = 1.00–1.44 (–1.57), av. Q=1.14, globose to subglobose, smooth, thin-walled. Basidia 4–spored, (25.0–) 33.0–48.0 × (5.2–) 5.8–7.4 μm, av. 40.1 × 6.5 μm, clavate, hyaline. Basidioles cylindrical to narrowly clavate, obtuse, (28.6–) 30.2–44.3 (–45.2) × (3.8–) 4.2–6.3 (–6.7) μm. Pleurocystidia absent. Pileipellis a hymeniderm to epithelium, hyphal terminations vertically arranged, composed of cells arranged in one or two ranks, terminal cells near the pileus margin obovoid, obpyriform, subglobose, clavate, thin-walled, (18–) 21–51 (–67) × (–15) 16–26 (–34) μm, av. 35 × 20 μm; subterminal cells usually distinctly narrower, cylindrical, some inflated, broadly clavate, ventricose or branched, (18–)20–46 (–50) × (6–) 8–19 (–23) μm, av. 29–13 μm. Terminal cells near the pileus center similar in shape and size to those near the pileus margin, but usually narrower, (15–) 17–34 (–40) × (–5) 7–14 (–20) μm, av. 23–11 μm, subterminal cells similar to those near the pileus margin, (6–) 7–23 (–34) × (1–) 2–5 (–10) μm, av. 16–6 μm. Caulocystidia without

pigments, thin walled, usually densely clustered, flexuous or spirally coiled, clavate and regular, (37–) 33–48 (–50) × (4.7–) 5.8–7.4 (–8.9) μm. Clamp connections absent in all parts. Ecology and Distribution: Solitary, on soil, in a forest dominated by Castanopsis. Known only from the type locality in Chiang Mai Province, Northern Thailand.

Additional specimen examined: Thailand, Chiang Mai Province, Wat Pah Pa Deng, Mae Taeng District, 14 Aug 2020, Feng-Ming Yu (MFLU21-0006); same location, 5 September 2020, Hong-Wei Shen (HKAS110665); Chiang Mai Province, Mae Sae Village, Pa Pae Sub-district, Mae Taeng District, 6 September 2020, Hong-Wei Shen

(HKAS110666).

Notes: Hodophilus pseudoglabripes is characterized by its slightly striate, flocculose pileus with white, yellowish white, greyish yellow and light orange colors, smooth white stipe, subglobose basidiospores, pileipellis terminal cells obpyriform, subglobose or clavate. Morphologically, H. tenuicystidiatus is similar to H. pseudoglabripes in their greyish yellow basidiomata. However, H. tenuicystidiatus has a smooth surface on the pileus, stipe usually grey orange to brown near lamellae and white tomentose at the base, and more lamellae number (20–34; Adamčík et al. 2017). Hodophilus phaeophyllus, H. stramineus, H. cambriensis and H. phaeoxanthus are similar to H. pseudoglabripes in their brownish yellow stipe. However, Hodophilus phaeophyllus pileus is greyish brown when fresh, hair brown when dry; and has smaller basidia (23.5–43 × 17.5–29.5 μm; Adamčík et al. 2020). Hodophilus stramineus has a greyish brown, brownish orange to grey brown pileus, relatively smaller basidiospores (4.1–4.7 × 3.3–3.8 μm) and wider caulocystidia (23.5–47 × 6–13.5 μm ; Adamčík et al. 2020). Hodophilus cambriensis stipe has very finely pruinous on the surface, pileus brownish grey and also with a yellow tint when fresh and moist, and has wider subterminal cells (26–43.5 × 18.5–30 μm; Adamčík et al. 2018). Hodophilus phaeoxanthus pileus yellowish brown to brown when fresh and moist, when dry near the margin, yellowish brown to brownish orange, basidiospores Qav=1.22, larger terminal cells (av.=34.8 × 23.5 μm) and subterminal cells (av.=17.9 × 7.6 μm; Adamčík et al. 2018). Hodophilus pseudoglabripes has a different odor from all the known Hodophilus species. In the phylogenetic tree, it is separated from all the other species. Hodophilus carpathicus is similar to H. pseudoglabripes in their globose to subglobose basidiospores. However, H. carpathicus has a coffee brown to chocolate pileus and more lamellae number (22–24), dark brown, coffee brown to mustard brown stipe and relatively wider basidia (32–42 × 6–7 μm; Adamčík et al. 2020). In our multi-locus phylogenetic analyses, H. pseudoglabripes is related to H. glabripes and H. indicus. However, H. glabripes has a relatively larger pileus (15–50 mm), which is non-striate when mature or indistinctly

translucently striate up to one third when wet and more lamellae (21–32; Zhang et al. 2019). Hodophilus indicus has smaller and brownish orange basidiomata, smaller basidiospores (4–5 × 3–5 μm), a solid and greyish orange stipe and an indistinctive odour.